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Isolation and molecular characterization of Bacillus thuringiensis found in soils of the Cerrado region of Brazil, and their toxicity to Aedes aegypti larvae Rev. Bras. entomol.
Lobo,Katiane dos Santos; Soares-da-Silva,Joelma; Silva,Maria Cleoneide da; Tadei,Wanderli Pedro; Polanczyk,Ricardo Antonio; Pinheiro,Valéria Cristina Soares.
ABSTRACT This study investigated the potential of Bacillus thuringiensis isolates obtained in the Cerrado region of the Brazilian state of Maranhão for the biological control of Aedes aegypti larvae. The isolates were obtained from soil samples and the identification of the B. thuringiensis colonies was based on morphological characteristics. Bioassays were run to assess the pathogenicity and toxicity of the different strains of the B. thuringiensis against third-instar larvae of A. aegypti. Protein profiles were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Polymerase chain reaction assays were used to detect the toxin genes found in the bacterial isolates. Overall, 12 (4.0%) of the 300 isolates obtained from 45 soil samples were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Entomopathogenic bacteria; Vector.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262018000100005
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Isolation of Bacillus thuringiensis from the state of Amazonas, in Brazil, and screening against Aedes aegypti (Diptera, Culicidae) Rev. Bras. entomol.
Soares-da-Silva,Joelma; Pinheiro,Valéria Cristina Soares; Litaiff-Abreu,Eleilza; Polanczyk,Ricardo Antonio; Tadei,Wanderli Pedro.
We investigated the use of Bacillus thuringiensis isolated in the state of Amazonas, in Brazil, for the biological control of the dengue vector Aedes aegypti. From 25 soil samples collected in nine municipalities, 484 bacterial colonies were obtained, 57 (11.78%) of which were identified as B. thuringiensis. Six isolates, IBt-03, IBt-06, IBt-07, IBt-28, IBt-30, and BtAM-27 showed insecticidal activity, and only BtAM-27 presents the five genes investigated cry4Aa, cry4Ba, cry10Aa, cry11Aa, and cry11Ba. The IBt-07 and IBt- 28, with lower LC50 values, showed equal toxicity compared to the standards. The isolates of B. thuringiensisfrom Amazonas constitute potential new means of biological control for A. aegypti, because of their larvicidal activity and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazonian; Bioinsecticide; Dengue; Vector.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262015000100001
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Oviposition of Aedes aegypti Linnaeus, 1762 and Aedes albopictus Skuse, 1894 (Diptera: Culicidae) under laboratory and field conditions using ovitraps associated to different control agents, Manaus, Amazonas, Brazil Rev. Bras. entomol.
Silva,William Ribeiro da; Soares-da-Silva,Joelma; Ferreira,Francisco Augusto da Silva; Rodrigues,Iléa Brandão; Tadei,Wanderli Pedro; Zequi,João Antonio Cyrino.
ABSTRACT The aim of this study was to analyze the effectiveness of different control agents of Aedes aegypti and Aedes albopictus associated with ovitraps under laboratory and field conditions. Five treatments were used: grass infusion + Bacillus thuringiensis israelensis (gI + Bti), grass infusion + Saccharopolyspora spinosa (gI + Ss), grass infusion + Pyriproxyfen (gI + P), distilled water + Toxorhynchites haemorrhoidalis (dW + Th), and grass infusion (gI) (control). The highest mean number of eggs of both species were obtained with grass infusion in the laboratory. Among control agents, the lowest mean of A. aegypti eggs occurred with gI + Ss and the lowest mean of A. albopictus eggs occurred with dW + Th. There was no difference between treatments in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chikungunya; Control; Dengue; Insecticides; Zika.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262018000400304
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